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plasmid dnas containing the brunello plasmid library  (Addgene inc)


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    Addgene inc plasmid dnas containing the brunello plasmid library
    Genome-wide CRISPR screen for essential hepatovirus host factors. a. Frequency distribution of the number of reads mapping to individual sgRNA integrants in high-throughput sequencing of control (GCV-treated) versus selected (18f-Tat virus-infected and GCV treated) HeLa-tkGFP cells transduced with lentiviruses expressing the <t>Brunello</t> sgRNA library in the first of two independent screens (screen #1). b. Number of reads (log 2 ) mapping to individual sgRNAs in control versus selected cells in screen #1. The total number of individual sgRNAs to which reads mapped is shown at the bottom. c. Scatterplot showing correlation of the positive selection scores accorded genes (n=19,111) on the basis of sgRNA enrichment in two independent screens. Red symbols indicate genes related to NeuNAc or ganglioside synthesis. Spearman’s rank correlation coefficient r =0.267, p < 0.0001. d. STRING analysis of functional associations of proteins encoded by genes comprising the top 39 hits identified in the combined analysis of the two independent screens. Common associations are annotated. Protein-protein interaction enrichment p-value = 3.11 ×10 −15 . e. Normalized counts of reads mapping to sgRNAs targeting selected genes involved in ganglioside synthesis (see main manuscript ) in control versus selected cell populations.
    Plasmid Dnas Containing The Brunello Plasmid Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+dnas+containing+the+brunello+plasmid+library/brunello+library+plasmid/pmc07483933-36-18-30
    Average 90 stars, based on 1 article reviews
    plasmid dnas containing the brunello plasmid library - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus"

    Article Title: Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus

    Journal: Nature microbiology

    doi: 10.1038/s41564-020-0727-8

    Genome-wide CRISPR screen for essential hepatovirus host factors. a. Frequency distribution of the number of reads mapping to individual sgRNA integrants in high-throughput sequencing of control (GCV-treated) versus selected (18f-Tat virus-infected and GCV treated) HeLa-tkGFP cells transduced with lentiviruses expressing the Brunello sgRNA library in the first of two independent screens (screen #1). b. Number of reads (log 2 ) mapping to individual sgRNAs in control versus selected cells in screen #1. The total number of individual sgRNAs to which reads mapped is shown at the bottom. c. Scatterplot showing correlation of the positive selection scores accorded genes (n=19,111) on the basis of sgRNA enrichment in two independent screens. Red symbols indicate genes related to NeuNAc or ganglioside synthesis. Spearman’s rank correlation coefficient r =0.267, p < 0.0001. d. STRING analysis of functional associations of proteins encoded by genes comprising the top 39 hits identified in the combined analysis of the two independent screens. Common associations are annotated. Protein-protein interaction enrichment p-value = 3.11 ×10 −15 . e. Normalized counts of reads mapping to sgRNAs targeting selected genes involved in ganglioside synthesis (see main manuscript ) in control versus selected cell populations.
    Figure Legend Snippet: Genome-wide CRISPR screen for essential hepatovirus host factors. a. Frequency distribution of the number of reads mapping to individual sgRNA integrants in high-throughput sequencing of control (GCV-treated) versus selected (18f-Tat virus-infected and GCV treated) HeLa-tkGFP cells transduced with lentiviruses expressing the Brunello sgRNA library in the first of two independent screens (screen #1). b. Number of reads (log 2 ) mapping to individual sgRNAs in control versus selected cells in screen #1. The total number of individual sgRNAs to which reads mapped is shown at the bottom. c. Scatterplot showing correlation of the positive selection scores accorded genes (n=19,111) on the basis of sgRNA enrichment in two independent screens. Red symbols indicate genes related to NeuNAc or ganglioside synthesis. Spearman’s rank correlation coefficient r =0.267, p < 0.0001. d. STRING analysis of functional associations of proteins encoded by genes comprising the top 39 hits identified in the combined analysis of the two independent screens. Common associations are annotated. Protein-protein interaction enrichment p-value = 3.11 ×10 −15 . e. Normalized counts of reads mapping to sgRNAs targeting selected genes involved in ganglioside synthesis (see main manuscript ) in control versus selected cell populations.

    Techniques Used: Genome Wide, CRISPR, Next-Generation Sequencing, Control, Virus, Infection, Transduction, Expressing, Selection, Functional Assay

    Related Articles

    Produced:

    Article Title: Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus
    Article Snippet: Anti-mouse horseradish peroxidase (HRP)-labeled secondary antibodies were from Southern Biotech (Cat# 1010–05) and IRDye 800CW donkey anti-goat IgG from Li-Cor Biosciences (Cat# 926–32214). .. A lentivirus pool was produced by transfection of HEK293 cells with a mix of plasmid DNAs containing the Brunello plasmid library , (a gift from David Root and John Doench, Addgene #73179), pVSVG (Addgene Cat#8454), and psPAX2 (Addgene Cat#12260) using the Trans-IT LT1 reagent (Mirusbio). ..

    Transfection:

    Article Title: Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus
    Article Snippet: Anti-mouse horseradish peroxidase (HRP)-labeled secondary antibodies were from Southern Biotech (Cat# 1010–05) and IRDye 800CW donkey anti-goat IgG from Li-Cor Biosciences (Cat# 926–32214). .. A lentivirus pool was produced by transfection of HEK293 cells with a mix of plasmid DNAs containing the Brunello plasmid library , (a gift from David Root and John Doench, Addgene #73179), pVSVG (Addgene Cat#8454), and psPAX2 (Addgene Cat#12260) using the Trans-IT LT1 reagent (Mirusbio). ..

    Plasmid Preparation:

    Article Title: Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus
    Article Snippet: Anti-mouse horseradish peroxidase (HRP)-labeled secondary antibodies were from Southern Biotech (Cat# 1010–05) and IRDye 800CW donkey anti-goat IgG from Li-Cor Biosciences (Cat# 926–32214). .. A lentivirus pool was produced by transfection of HEK293 cells with a mix of plasmid DNAs containing the Brunello plasmid library , (a gift from David Root and John Doench, Addgene #73179), pVSVG (Addgene Cat#8454), and psPAX2 (Addgene Cat#12260) using the Trans-IT LT1 reagent (Mirusbio). ..



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    plasmid dnas containing the brunello plasmid library  (Addgene inc)
    90
    Addgene inc plasmid dnas containing the brunello plasmid library
    Genome-wide CRISPR screen for essential hepatovirus host factors. a. Frequency distribution of the number of reads mapping to individual sgRNA integrants in high-throughput sequencing of control (GCV-treated) versus selected (18f-Tat virus-infected and GCV treated) HeLa-tkGFP cells transduced with lentiviruses expressing the <t>Brunello</t> sgRNA library in the first of two independent screens (screen #1). b. Number of reads (log 2 ) mapping to individual sgRNAs in control versus selected cells in screen #1. The total number of individual sgRNAs to which reads mapped is shown at the bottom. c. Scatterplot showing correlation of the positive selection scores accorded genes (n=19,111) on the basis of sgRNA enrichment in two independent screens. Red symbols indicate genes related to NeuNAc or ganglioside synthesis. Spearman’s rank correlation coefficient r =0.267, p < 0.0001. d. STRING analysis of functional associations of proteins encoded by genes comprising the top 39 hits identified in the combined analysis of the two independent screens. Common associations are annotated. Protein-protein interaction enrichment p-value = 3.11 ×10 −15 . e. Normalized counts of reads mapping to sgRNAs targeting selected genes involved in ganglioside synthesis (see main manuscript ) in control versus selected cell populations.
    Plasmid Dnas Containing The Brunello Plasmid Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+dnas+containing+the+brunello+plasmid+library/brunello+library+plasmid/pmc07483933-36-18-30
    Average 90 stars, based on 1 article reviews
    plasmid dnas containing the brunello plasmid library - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Genome-wide CRISPR screen for essential hepatovirus host factors. a. Frequency distribution of the number of reads mapping to individual sgRNA integrants in high-throughput sequencing of control (GCV-treated) versus selected (18f-Tat virus-infected and GCV treated) HeLa-tkGFP cells transduced with lentiviruses expressing the Brunello sgRNA library in the first of two independent screens (screen #1). b. Number of reads (log 2 ) mapping to individual sgRNAs in control versus selected cells in screen #1. The total number of individual sgRNAs to which reads mapped is shown at the bottom. c. Scatterplot showing correlation of the positive selection scores accorded genes (n=19,111) on the basis of sgRNA enrichment in two independent screens. Red symbols indicate genes related to NeuNAc or ganglioside synthesis. Spearman’s rank correlation coefficient r =0.267, p < 0.0001. d. STRING analysis of functional associations of proteins encoded by genes comprising the top 39 hits identified in the combined analysis of the two independent screens. Common associations are annotated. Protein-protein interaction enrichment p-value = 3.11 ×10 −15 . e. Normalized counts of reads mapping to sgRNAs targeting selected genes involved in ganglioside synthesis (see main manuscript ) in control versus selected cell populations.

    Journal: Nature microbiology

    Article Title: Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus

    doi: 10.1038/s41564-020-0727-8

    Figure Lengend Snippet: Genome-wide CRISPR screen for essential hepatovirus host factors. a. Frequency distribution of the number of reads mapping to individual sgRNA integrants in high-throughput sequencing of control (GCV-treated) versus selected (18f-Tat virus-infected and GCV treated) HeLa-tkGFP cells transduced with lentiviruses expressing the Brunello sgRNA library in the first of two independent screens (screen #1). b. Number of reads (log 2 ) mapping to individual sgRNAs in control versus selected cells in screen #1. The total number of individual sgRNAs to which reads mapped is shown at the bottom. c. Scatterplot showing correlation of the positive selection scores accorded genes (n=19,111) on the basis of sgRNA enrichment in two independent screens. Red symbols indicate genes related to NeuNAc or ganglioside synthesis. Spearman’s rank correlation coefficient r =0.267, p < 0.0001. d. STRING analysis of functional associations of proteins encoded by genes comprising the top 39 hits identified in the combined analysis of the two independent screens. Common associations are annotated. Protein-protein interaction enrichment p-value = 3.11 ×10 −15 . e. Normalized counts of reads mapping to sgRNAs targeting selected genes involved in ganglioside synthesis (see main manuscript ) in control versus selected cell populations.

    Article Snippet: A lentivirus pool was produced by transfection of HEK293 cells with a mix of plasmid DNAs containing the Brunello plasmid library , (a gift from David Root and John Doench, Addgene #73179), pVSVG (Addgene Cat#8454), and psPAX2 (Addgene Cat#12260) using the Trans-IT LT1 reagent (Mirusbio).

    Techniques: Genome Wide, CRISPR, Next-Generation Sequencing, Control, Virus, Infection, Transduction, Expressing, Selection, Functional Assay